Towards the elimination of ergot alkaloid biosynthesis genes in Neotyphodium Coenophialum

An ergot alkaloid biosynthesis gene and clustered hypothetical genes from Aspergillus fumigatus.

The ergot alkaloids are a family of indole-derived mycotoxins with a variety of significant biological activities. Aspergillus fumigatus, a common airborne fungus and opportunistic human pathogen, and several fungi in the relatively distant taxon Clavicipitaceae (clavicipitaceous fungi) produce different sets of ergot alkaloids. The ergot alkaloids of these divergent fungi share a four-member e...

Progress and prospects in ergot alkaloid research.

Ergot alkaloids, produced by the plant parasitic fungi Claviceps purpurea are important pharmaceuticals. The chemistry, biosynthesis, bioconversions, physiological controls, and biochemistry have been extensively reviewed by earlier authors. We present here the research done on the organic synthesis of the ergot alkaloids during the past two decades. Our aim is to apply this knowledge to the sy...

Loline Alkaloid Gene Clusters of the Fungal Endophyte Neotyphodium Uncinatum

Progress and Prospects of Ergot Alkaloid Research

Ergot alkaloids, produced by the plant parasitic fungi Claviceps purpurea are important pharmaceuticals. The chemistry, biosynthesis, bioconversions, physiological controls, and biochemistry have been extensively reviewed by earlier authors. We present here the research done on the organic synthesis of the ergot alkaloids during the past two decades. Our aim is to apply this knowledge to the sy...

Ergot alkaloid biosynthesis in Aspergillus fumigatus: overproduction and biochemical characterisation a 4-dimethylallyltryptop

A putative gene fgaMT was identified in the biosynthetic gene cluster of fumigaclavines in Aspergillus fumigatus. The coding region of fgaMT was amplified by PCR from a cDNA library, cloned into pQE60 and overexpressed in E. coli. FgaMT comprises 339 amino acids with a molecular weight of about 38.1 kDa. The soluble dimeric His6-FgaMT was purified to near homogeneity and characterised biochemic...